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  • 08월 28일 16시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

Separation of Specific DNA Fragments for Fast Detection by Microchip Electrophoresis Using Programmed Field Strength Gradient

등록일
2008년 8월 12일 08시 48분 32초
접수번호
0978
발표코드
금13H7심 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
발표시간
금 11시 : 10분
발표형식
심포지엄
발표분야
분석화학 - Novel Methods in Separation Science
저자 및
공동저자
강성호
전북대학교 화학과, Korea
We evaluated a novel strategy for fast detection of specific DNA fragments by microchip electrophoresis (ME) using programmed field strength gradients (PFSG) in a conventional glass double-T microchip. The ME-PFSG allows for the ultra-fast separation and enhanced resolving power for target DNA fragments. These results are based on electric field strength gradients (FSG) that use an ME separation step in a sieving gel matrix. The gradient can develop staircase or programmed shapes FSG over the time. The PFSG method could be easily used to increase separation efficiency and resolution in ME separation of specific size DNA fragments. Compared to ME that uses a conventional and constantly applied electric field method, the ME-PFSG achieved about 15-fold faster analysis time during the separation of 100 bp DNA ladder. The ME-PFSG was also applied to the ultra-fast analysis for specific DNA fragments of various genetically modified organisms in maize and soybeans as well as fast diagnosis without the loss of resolving power and reproducibility.

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