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Inhibition mechanism of Curcumin on the binding of bZIP transcription factor and DNA

등록일
2005년 2월 17일 16시 45분 23초
접수번호
1038
발표코드
23P172포 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
발표시간
금 <발표Ⅱ>
발표형식
포스터
발표분야
생명화학
저자 및
공동저자
이용찬, 한효규1, 최무현
고려대학교 생명과학대학,
1고려대학교 화학과,
Curcumin, a natural spice, was found to possess chemopreventive and anticarcinogenic effect. It interferes binding between transcription factor and target site directly and inhibits the expression of many genes that are closely related on cellular proliferation, differentiation, and tumorigenesis. To elucidate the inhibition mechanism of curcumin, we synthesized curcumin analogs and performed electrophoretic mobility shift assay using synthetic oligonucleotide (AP-1 DNA binding site) and the basic region-leucine zipper (bZIP) domain of GCN4, GCN4-bZIP, expressed in Escherichia coli. The stability of curcumin derivatives was checked by UV abosorption. The anticancer activity to the various cancer cell lines was obtained by cytotoxicity assay. The inhibition effect of curcumin and its derivatives is strongly dependent on the hydroxyl group of the two aromatic rings of curcumin. According to the stability assay and cytotoxicity assay, we obtained a analog that is stable in aqueous solution and shows anticancer activity equivalent to curcumin.

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