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  • 08월 28일 16시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

Purification of a neuropeptide with contractile activity from squid brain,(Todarodes pacificus) [우수포스터상-생명화학]

등록일
2008년 8월 12일 12시 00분 50초
접수번호
1317
발표코드
33P228포 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
발표시간
목 <발표Ⅰ>
발표형식
포스터
발표분야
생명화학
저자 및
공동저자
고혜진, 조은희, 문지영, 권민희, 김혜림, 박남규
부경대학교 생물공학과, Korea
Neuropeptides act as intercellular signalling molecules in the nervous systems of animals and are involved in the regulation of numerous physiological processes. A neuropeptide with contractile activity on the squid digestive gland was isolated from the brain of squid. (Todarodes pacificus) The acetic acid extracts of squid gill were forced through the Sep-pak C18 cartridge devided by flow through(F.T), 0% methanol-elute(D.W), 60%methanol-elute(RM60), 100%methanol-elute(RM100). The activity was estimated by concentration of 1/4000. Among of them, the RM60 showed the best contractile activity on the squid digestive gland. Then RM60 was purified by C18 reversed phase and ion-exchange HPLC. The contractile peptide was obtained in isocratic condition 18% CH3CN in 0.1% TFA (pH2.2). At present, to analyze the primary structure of this peptide, automated amino acid sequencing is in progress.

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