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  • 08월 28일 16시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

Immobilization of fluorescent tagged DNA on PDMS microchip

등록일
2008년 8월 12일 14시 58분 33초
접수번호
1488
발표코드
32P124포 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
발표시간
금 <발표Ⅳ>
발표형식
포스터
발표분야
분석화학
저자 및
공동저자
구진아, 장지희, 주재영1, 임흥빈
단국대학교 화학과, Korea
1단국대학교 자연과학부 화학과, Korea
Fluorescent tagged DNA was immobilized on PDMS for microfluidic system. For the immobilization, 3-(aminopropyl)triethoxysilane(APTES) was deposited on the PDMS surface by CVD process after oxygen plasma treatment, in which covalently bonded amine funtionalized silicon elastomer was made. Then, sulfosuccinimidyl-4-(N-maleimidomethly)cyclohexane-1-carboxylate(Sulfo-SMCC) was attached on the amine surface as a crosslinker before the surface was coated with containing single-DNA in terms of thiol reactivity. The cross linker was efficiently identified by FT-IR and the single strand DNA containing FITC can be monitored by laser induced fluorescence microscope. Furthermore, the application of this system will be presented in detail.

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