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  • 08월 28일 16시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

Determination of Zn2+ binding affinity of peptides using simple ESI-MS Titration

등록일
2008년 8월 22일 19시 49분 28초
접수번호
1587
발표코드
목12H1심 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
발표시간
목 14시 : 00분
발표형식
심포지엄
발표분야
분석화학 - Mass spectrometry for chemical analysis
저자 및
공동저자
오한빈
서강대학교 화학과, Korea

Metalloproteins participate in many important biological processes, of which metal-binding sites are often located at the active site. In particular, a Zinc ion is essential as a structural factor of zinc finger proteins which constitute one of the most common DNA binding motif. In therapeutics, there have been extensive efforts to artificially design new types of Zinger finger proteins and also to develop a method which can facilitate the screening of the designed proteins. The main target of the screening has been their binding affinity to the targeted DNAs. Traditionally, spectroscopic tools, such as CD, NMR, and fluorescence measurements, have been successfully used. In our study, we tested the applicability of the electrospray ionization mass spectrometry as an alternative tool for the screening purpose. We also determined the Zn2+ binding affinity for a variety of artificially designed peptides. In the symposium, our recent results and its potential applications will be presented.


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