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Nanocatalyst-Based Assay Using DNA-Conjugated Au Nanoparticles for Electrochemical DNA Detection

등록일
2009년 2월 9일 13시 24분 06초
접수번호
0159
발표코드
38P278포 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
발표시간
금 <발표Ⅲ>
발표형식
포스터
발표분야
전기화학
저자 및
공동저자
허찬화, Das Jagotamoy, 양해식
부산대학교 화학과, Korea
Au nanoparticles (AuNPs) are used as catalytic labels to achieve ultrasensitive DNA detection via fast catalytic reactions. In addition, magnetic beads (MBs) are employed to permit low nonspecific binding of DNA-conjugated AuNPs and to minimize electrocatalytic current of AuNPs as well as to take advantage of easy magnetic separation. In a sandwich-type electrochemical sensor, a thiolated detection-probe-conjugated AuNP exhibits a high level of unblocked active sites and permits the easy access of p-nitrophenol and NaBH4 to these sites. Electroactive p-aminophenol is generated at these sites and is then electrooxidized to p-quinoneimine at the electrode. The p-aminophenol redox cycling by NaBH4 offers large signal amplification. The nonspecific binding is lowered by washing DNA-linked MB-AuNP assemblies with a formamide-containing solution. The high signal amplification and low background current enable the detection of 1 fM target DNA.

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