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  • 02월 23일 15시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

Comparison of the Nonspecific Binding of DNA-Conjugated Gold Nanoparticles between Polymeric and Monomeric Self-Assembled Monolayers

등록일
2009년 2월 9일 13시 38분 11초
접수번호
0164
발표코드
38P264포 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
발표시간
금 <발표Ⅲ>
발표형식
포스터
발표분야
전기화학
저자 및
공동저자
Das Jagotamoy, 양해식
부산대학교 화학과, Korea
The nonspecific binding of DNA-conjugated gold nanoparticles (AuNPs) to solid surfaces is more difficult to control than that of DNA molecules due to the more attractive interactions from the large number of DNA molecules per AuNP. We report that the polymeric self-assembled monolayers (SAMs) formed on indium-tin oxide (ITO) electrodes significantly inhibit the nonspecific binding of DNA-conjugated AuNPs. The random copolymers used to prepare the polymeric SAMs consist of three functional parts: an ITO-reactive silane group, a DNA-blocking poly(ethylene glycol) (PEG) group, and an amine-reactive N-acryloxysuccinimide group. We compare the nonspecific binding of DNA-conjugated AuNPs between the polymeric SAMs and various monomeric SAMs. Carboxylicacid-terminated and amine-terminated monomeric SAMs cause high levels of nonspecific binding of DNA-conjugated AuNPs. The monomeric SAM modified with the carboxylic-acid-terminated poly(amidoamine) dendrimer shows low levels of nonspecific binding (2.0% nonspecific binding) due to the high surface density of the negative charge. The simply prepared polymeric SAM produces the lowest level of nonspecific binding (0.8% nonspecific binding), resulting from the combined effect of (i) DNA-blocking PEG and carboxylic acid groups and (ii) dense polymeric SAMs.

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