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High Throughput Proteome Analysis with Data Validation by Multidimensional Separation and High Resolution Mass Spectrometry

등록일
2005년 2월 25일 18시 17분 11초
접수번호
1095
발표코드
금14D3심 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
발표시간
금 15시 : 00분
발표형식
심포지엄
발표분야
분석화학 - 최근 질량분석학의 발전
저자 및
공동저자
유종신
한국기초과학지원연구원,
Proteomics deals with the large-scale determination of cellular function directly at the protein level. By the nature of the complexity of cellular proteomes, which contain thousands of proteins over a wide dynamic range of abundance, it is recommended to have a multidimensional type of analytical tools. A various fractionation and analysis method have to be used to extend the range and total number of proteins identified with high confidence. This presentation will show the high throughput proteome analysis using multidimensional separation and tandem mass spectrometry at the protein and peptide level. The proteins separated by molecular weight were fractionated into gel bands and in-gel digested with trypsin. The resulting peptides were analyzed by LC/ESI-MS/MS and MALDI-MS/MS with a strong cation exchange and reversed-phase columns packed in series to separate the peptides. To eliminate false-positive proteins from the database search, we employed a protein data-validation method based on molecular weight correlation from one-dimensional gel electrophoresis. The proposed approach was proven to be effective for complex proteome analysis of human and pseudomonas on a large scale, which resulted the complementary information both from LC-MALDI-MS/MS with TOF/TOF and LC/ESI-MS/MS analysis with 7T FT Mass Spectrometry..

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