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초록문의 abstract@kcsnet.or.kr

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  • 02월 23일 15시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

Cell based in-vitro sensor systems for monitoring of free radical production and the activation of neuronal nitric oxide synthase.

등록일
2009년 2월 12일 14시 43분 48초
접수번호
1201
발표코드
금19F4심 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
발표시간
금 12시 : 00분
발표형식
심포지엄
발표분야
전기화학 - Young Electrochemist Symposium II
저자 및
공동저자
장승철
부산대학교 신개념바이오피지오센서기술연구센터, Korea
Electrochemical cell-based sensor systems for the simultaneous monitoring of extracellular production of biologically important free radical species has been developed and assessed using an array-based cell chip approach. The present model system has been used to evaluate extracellular superoxide (O2¯) and nitric oxide (NO) production by a stimulated glioblastoma multiform cell line (A172). Extracellular O2¯ and NO were detected simultaneously by using an amperometric sensor constructed by immobilization of cytochrome c, to attach the redox protein onto the surface of electrodeposited Au electrodes incorporated into the cell chip. A solid-contact potentiometric immunosensor has also been developed to study the activation process of neuronal nitric oxide synthase (nNOS), an enzyme involved in the synthesis of nitric oxide which is a free radical generated under physiological conditions by virtually all mammalian cells. Simple drop-coating of PVC carboxylated ionophore was performed on the electrode, forming a strongly-attached polymer film on the electrode surface. The secondary antibody-urease conjugate and anti-phospho-nNOS antibody-antigen interaction alter the potential and interfacial electron transfer resistance of the electrode. The results obtained indicated that there was no detectable interference or crosstalk between the electrochemical measurement s and the response time of the sensor system was found to be in the range of a few tens of seconds. The activation and inhibition processes of nNOS were successfully observed in real samples from A172 cells. A full description of the system development and performance will be given in this presentation.

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