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Separation of PAMAM dendrimers of various generations by using Asymmetrical Flow Field-Flow Fractionation (AsFlFFF)

등록일
2009년 8월 14일 14시 32분 03초
접수번호
1061
발표코드
35P99포 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
발표시간
금 <발표Ⅳ>
발표형식
포스터
발표분야
분석화학
저자 및
공동저자
Sachin Nehete
한남대학교 화학과, Korea
Asymmetrical-flow field flow fractionation (AsFlFFF) was successfully applied for separation of polyamidoamine (PAMAM) dendrimers of various generations (G4-G9), which have molecular weights ranging in 14-467 KDa and the radius of gyrations ranging in 17.87-46.47Å. A wide variety of analytical techniques have been tested for characterizing dendrimers: size exclusion chromatography (SEC), low angle laser light scattering, infrared (IR) spectroscopy, capillary electrophoresis, Mass spectrometry (chemical ionization and fast-atom bombardment), laser desorption, electro-spray ionization, matrix-assisted laser desorption/ionization-time-of-flight (MALDI-TOF), and 1H, 2H, 13C NMR. The performance of all those techniques tends to decline as the size of the dendrimer increases (1). It was reported that in SEC at neutral pH (7.2) due to strong electrostatic interaction with the column packing material, dendrimers (Generation-5) were not able to elute from SEC columns.(2) AsFlFFF, due to its open channel geometry, was able to separate and characterize the dendrimers without sample degradation. AsFlFFF was also applied to study the interaction of the PAMAM dendrimer (generation-5) and bovine serum albumin (BSA). It was found that higher order aggregates were formed at relatively higher dendrimer concentration. Results suggest AsFlFFF is useful for the separation and characterization of large charged molecules such as PAMAM dendrimers.

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