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DNA cleavage by autoxidation of Fe ions
2009년 8월 14일 15시 58분 56초
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영남대학교 화학과, Korea
Iron ions are ubiquitous and critical in the cell metabolism, but it has been proposed that they can damage to various biological components including DNA. The well-known mechanism for iron-induced DNA cleavage reaction is “Fenton reaction” in which Fe(II) reacts with H
to produce highly reactive •OH and related oxidants. Subsequently, •OH induces DNA cleavage by proton subtraction via attacking sugar or by hydroxylation to the DNA base. Alternatively, another pathway of iron-induced cleavage has been proposed that iron ions alone can generate DNA cleavage in the absence of H
. In this study, we are investigating the properties and the mechanism of iron ions induced DNA cleavage reaction. First, we compared the cleavage reaction with Fe(II) and Fe(III) ions. Fe(III) ion also exhibited plasmid DNA degradation although the efficiency was much lower than that by Fe(II). However, it seemed that Fe(II) and Fe(III) may follow different mechanism according to the pH dependent cleavage reaction. N
bubbling diminished the Fe(II)-induced plasmid cleavage efficiency, indicating that Fe(II) may use oxygen molecules to produce reactive molecular species. In the compare of the Fe(II)-induced reaction in the presence and absence of H
, the cleavage efficiency was much higher with Fe(II) alone than that in the presence of H
. On the other hand, decrease of cleavage ability of Fe(II) upon increase of H2O2 indicates that there might be a competition for Fe(II) to oxygen molecules and H
2. In the experiment with various radical scavengers, it seems that several radical species such as singlet oxygen, superoxide radical as well as •OH participate in the iron ions induced DNA cleavage reaction.
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