abs

학술발표회초록보기

초록문의 abstract@kcsnet.or.kr

결제문의 member@kcsnet.or.kr

현재 가능한 작업은 아래와 같습니다.
  • 09월 04일 13시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

Direct Label-Free Electrical Immunodetection of Biomarkers in Human Serum

등록일
2009년 8월 14일 18시 55분 36초
접수번호
1326
발표코드
금13E1심 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
발표시간
금 09시 : 00분
발표형식
심포지엄
발표분야
분석화학 - Development and Applications of Analytical Chemistry in Bio- and Nano-Technologies
저자 및
공동저자
김안순, 아칠성
한국전자통신연구원 바이오센서연구팀, Korea

Simple and fast biomarker screens have a central role in many areas of healthcare, including disease diagnosis and drug discovery. Biologically modified field-effect transistor (BioFET) is one of the most attractive approaches because of the on-chip integration of the sensor array, fast response, high reliability and low-cost mass production. However, the BioFETs used to detect macromolecules have been operated only in buffer solution with low salt concentrations because of the Debye screening length of blood or serum. Here we report a novel detection technique for direct label-free immunodetection of cancer markers in human serum using a Si-FET that was fabricated by conventional photolithographic processes. The proposed method that is sensing in low salt buffer after immunoreaction in serum overcomes the Debye length problem of the BioFETs, and have been confirmed by using sandwich immunoassay with immunogold nanoparticles. Our results demonstrate specific label-free and real-time detection of cancer markers at a concentration of 0.2 ng/mL in human serum, and successful quantitative and multiplexed sensing.


상단으로