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Direct, Site-specific Immobilization of natural sugar on Hydrazide coated glass slide

등록일
2009년 8월 21일 15시 41분 46초
접수번호
1541
발표코드
37P97포 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
발표시간
금 <발표Ⅲ>
발표형식
포스터
발표분야
유기화학
저자 및
공동저자
박성진
연세대학교 화학과, Korea
The understanding of glycan-protein interactions at the molecular level provides deep insight into glycan-mediated biological processes and enables the development of more effective therapeutic agents and diagnostic tools in the post-genomics era. Carbohydrate microarrays, which are composed of diverse glycans orderly and densely attached to a single chip substrate, have been extensively developed as reliable and efficient tools for the rapid analysis of carbohydrate-based biomolecular interactions. The most general method for construction of these microarrays involves site-specific and covalent immobilization of chemically modified carbohydrates to properly derivatized surfaces. This immobilization strategy requires the availability of modified glycans whose syntheses in many cases are time-consuming and difficult. In recent investigations directed at this goal, we have developed a novel, direct, site-specific technique for immobilization of unmodified carbohydrates, including simple carbohydrates, oligosaccharides and polysaccharides, on hydrazide-derivatized surfaces. This method eliminates the need for laborious and tedious glycan derivatization. To demonstrate the scope and applicability of this approach, carbohydrate microarrays containing a variety of glycans (fifty eight glycans) have been constructed by using one-step, direct attachment of free carbohydrates to a hydrazide-coated surface. These microarrays have been employed for analysis of sugar binding specifities of lectins, antibodies and bacterial cells. In addition, this microarray format has been applied to the determinination of binding affinities between proteins and glycans.

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