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Effect of Heavy Metal on Single Strain DNA immobilized on PDMS microchip and dye doped silica bead

등록일
2009년 8월 21일 15시 57분 26초
접수번호
1548
발표코드
35P142포 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
발표시간
금 <발표Ⅳ>
발표형식
포스터
발표분야
분석화학
저자 및
공동저자
구진아, 고정아, 임흥빈
단국대학교 화학과, Korea
Recently, we have been exposed to many pollutants, for example, heavy metals, ultraviolet, chemicals, and so on. Particularly, heavy metals like Hg, Cd, Pb accumulated in organ such as lung and kidney, occur many symptoms. In this experiment, the cause of diseases due to the exposure was studies in the point of view of the reaction between DNA with heavy metals. Two different model systems, PDMS microarray-chip and FITC doped silica beads were setup for the reaction. For the PDMS microarray chip, fluorophore-tagged DNA was immobilized on the chip through the attachment of linker. In case of silica beads, about 50 ~ 80nm size of the beads doped with FITC(fluorescein isothiocyanate, Emax for absorption=494nm, Emax for emission=521nm) were synthesized by microemulsion method. Before metal reaction silica beads of NH2 modified and one end cy5, the the other end carboxylic acid labeled DNA reacted, then checked the reaction of fluorescent-tagged DNA with several heavy metals(Cd,Pb) by determining the fluorescent emission cleaved from the DNA immobilized chip or silica beads. For quantitative measurement, fluorescence quenching due to the presence of metal was studied as well.

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