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DNA Minor Groove Binders interaction

등록일
2009년 8월 21일 17시 10분 40초
접수번호
1560
발표코드
34P170포 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
발표시간
목 <발표Ⅱ>
발표형식
포스터
발표분야
물리화학
저자 및
공동저자
김우성
고려대학교 화학과, Korea
Diatamycin A(DST) and Netropsin(NET) are a well known polyamide antibiotic that can bind in the minor groove of duplex DNA primarily at AT-rich sequences. An FTIR and CD study of the DNA binding modes of DST and NET were investigated binding studies with oligomeric duplexes of the form d(CGCGAAAAACGCG)·(CGCGTTTTTCGCG). Distamycin A can bind both as a monomer or as a side-by-side antiparallel dimer. In case of oligomer duplexes with AAAAA binding sites,1:1 complexes are formed at low distamycin:DNA ratios and 2:1 complexes are formed at the ratios >1. It was found that 1:1 and 2:1 drug-duplex complexes exhibit distinctly different circular dichroic(CD) spectral characteristics and can, thus, serve as diagnostic tools for binding mode differentiation. It the infrared spectra of antiparallel-stranded(aps) DNA have been studied. Significant differences have been found in the region of the thymine C=O stretchingvibrations. Interaction with three drugs known to bind in the minor groove of aps-DNA - netropsin and distamycin A - induces shifts of the C = O stretching vibrations of ps-DNA even at low ratio of drug per DNA base pair.

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