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Electrochemical Assay of Adenosine Based on Guanine Bases of an Adenosine-sensitive Aptamer

2009년 8월 26일 14시 02분 48초
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금 <발표Ⅲ>
저자 및
최민선, Wu Qiong, 이성은, 김진흥
이화여자대학교 나노과학부, Korea
Aptamers as the active sensing materials are synthetic oligonucleotides forming characteristic tertiary structures, such as G-quartet and hairpin, upon binding to specific proteins, small molecular targets, and metal ions. Up to now, various techniques, such as colorimetry, fluorescence, and electrochemistry, have been used for the detection of thrombin, adenosine, cocaine, and potassium ions as the targets using the corresponding aptamer probes. As a result of their high selectivity, stability, target versatility, and convenient regeneration and modification, aptamers hold great promise for various diagnostic applications. Recently, sensing of small molecules and ions coupling the attractive properties of aptamers has received considerable attention. Electrochemistry offers attarctive routes for interfacing aptamer interactions with the signal generating element in a simple, fast, and inexpensive manner. Modern electronic aptamer biosensors commonly rely on redox tags with conformational changes, or impedance detection to offer convenient measurements of nanomolar concentrations of target molecules. Further enhancement of the sensitivity of such devices is urgently needed for measuring ultra low levels of target agents. Recent studies to improve the sensitivity of aptamer-based biosensors and bioassays have lowered their detection limits to the picomolar level through the use of nanoparticle tags, binding-induced conformational changes, and label-free impedance detection. We herein present an electrochemical aptamer-based sensors with metal complexes as mediators utilizing a specific interaction between DNA and metal complex.