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Chemical Approach for Specific Enrichment and Mass Analysis of Nitrated Peptides

등록일
2009년 9월 8일 17시 52분 44초
접수번호
1614
발표코드
금13E6심 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
발표시간
금 11시 : 20분
발표형식
심포지엄
발표분야
분석화학 - Development and Applications of Analytical Chemistry in Bio- and Nano-Technologies
저자 및
공동저자
Kwang Pyo Kim, Woon-Seok Yeo
Department of Molecular Biotechnology, Konkuk University, Seoul 143-701, Korea, Korea
The analysis and detection of 3-nitrotyrosine are biologically and clinically important because protein tyrosine nitration is known to be involved in a number of biological phenomena such as cellular signal transduction, pathogenesis of inflammatory responses, and age-related disorders. However, the main obstacles in the study are low abundance of nitrated species and lack of efficient enrichment methods. Here in, we introduce new chemical approaches to analyze nitrated peptides using mass spectrometry by incorporating specific tagging groups in the peptides through simple chemical transformations. We further demonstrated the utility of our approaches by detection of nitrated peptides in complex samples such as tryptic peptide mixtures of bovine serum albumin (BSA), Huh7 cell lysate and HeLa cell lysate.

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