KCS

The amperometric detection methods of chemical species, especially metal ions and biomolecules, using various elelectrodes will be broadly discussed. First, for the amperometric detection of the heavy metal ions, the anodic stripping volammetric technique can be selected as a very sensitive tool. For example, this traditional electrochemical technique was implemented to make a miniaturized low-power wireless remote environmental monitoring system with the help of modern semiconductor technique. Secondly, the enzyme-amplified electrochemical detection method was recently developed for the sensitive detection of biomolecules such as DNA and protein. We will discuss how to generate the enzyme-amplified amperometric signal caused by the DNA hybridization reaction or antigen-antibody interaction. For the enhanced detection of biomolecules, we developed a method combining the old and new techniques, namely anodic stripping voltammetry and enzyme-amplified electrochemical detection method. In brief, the silver metal was deposited by the immobilized enzyme with specific interaction of biomolecules at the modified electrode. When the accumulated silver metal is stripped out with anodic potential scan, the anodic current signal allows us the ultrasenstitive detection of biomolecules.