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대한화학회 제105회 학술발표회 및 총회 Mesoporous Hollow Silica Capsules with a Large Pore Opening: Intracellular Delivery of Membrane-Impermeable Proteins, Molecules and Ions

2010년 2월 12일 20시 12분 36초
39P186포 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
금 <발표Ⅳ>
저자 및
허성, 임지선, 이기원1, 황용경, 김성진2, 권혁만1
한국외국어대학교 화학과, Korea
1한국외국어대학교 생명공학과, Korea
2이화여자대학교 화학과, Korea

Mesoporous rods and spheres with suitable pore dimensions and organic functionalities have been extensively prepared and employed in a variety of biological applications such as bioimaging and delivery. Both shape and organic functionality on the surface of mesoporous silica nanoparticles (MSNs) played an important role in the efficacy of small drug molecule delivery into HeLa cells. Here we report our new preparation method of hollow mesoporous silica capsules with a large pore opening. Pure siliceous capsule, carboxy-capsule, and amino-capsule were successfully prepared and characterized by gas sorption, TEM, SEM, and PXRD. Due to the large pore opening, the mesoporous capsules are suited for the intracellular delivery of proteins as well as various cell membrane-impermeable molecules and ions. The CLSM result, TEM and FACS data clearly support their efficient transport into HeLa cells. Compared with pure siliceous capsule, the amino-capsule exhibited almost no cytotoxicity for a prolonged incubation period supported by WST-1 assay tests and cell proliferation experiments conducted for 96 h. It was also possible to functionalize the surface of carboxy-capsule with other amine-containing molecules for further applications. For example, 5-aminofluorescein could be successfully attached on the surface of carboxy-capsule through a simple amidation. Green fluorescent protein (GFP) delivery will also be demonstrated.