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제105회 대한화학회 학술발표회, 총회 및 기기전시회 안내 Two-Photon Zinc Selective Probes with Various Affinities for Imaging in Live Cells and Tissues

등록일
2010년 2월 24일 16시 16분 51초
접수번호
1337
발표코드
37P264포 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
발표시간
금 <발표Ⅲ>
발표형식
포스터
발표분야
유기화학
저자 및
공동저자
Isravel Antony Danish, 임창수1, 정철1, 한지희1, 김창우1, 황혜림1, 조봉래1
KOREA UNIVERSITY, India
1고려대학교 화학과, Korea

We report a series of fluorescent two photon Zn2+ selective sensor molecules, AZNM1-AZNn with different dissociation constants, because the concentration of Zn2+ in biological system differs over a wide range from sub-nanomolar to millimolar. Thus prepared sensors have Kd values in the range of 10-9-10-5 M, compared with 0.5 nM for previously reported AZn2. All the compounds were having a solubility of 3.0 µM and are not affected by the presence of other biologically important metal ions such as calcium or magnesium, they can detect Zn2+ within 100 ms. The bright field image of a part of fresh hippocampal slices incubated with 10 mM AZnM2, AZnE1, AZnE2, and AZnN revealed the CA1 and CA3 regions as well as the dentate gyrus (DG). The TPM images AZnM2-labeled tissue clearly revealed the Zn2+ distribution of the same regions at 80-170 μm depth Thus, by using a combination of sensor molecules, it was shown that a higher Zn2+ concentration is released in DG than in CA3 or CA1 and that we can easily visualize Zn2+ concentration over a wide range. We has shown that these probes are highly suitable to detect Zn2+ in live cells and living tissues at 100 μm depth with high resolution and without mis-targeting.


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