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  • 08월 24일 19시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

Expression and purification of PKC isoforms in E.coli and their usefulness

등록일
2005년 8월 10일 16시 09분 46초
접수번호
0476
발표코드
24P125포 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
발표시간
금 <발표Ⅰ>
발표형식
포스터
발표분야
생명화학
저자 및
공동저자
김동희, 소재원
인하대학교 화학과,
Protein kinase C (PKC) represents a family of at least 11 serine/threonine kinase that participate in signal transduction events in response to specific hormonal, neuronal and growth factor stimuli. To examine roles of individual isoforms of PKCs, we used His-tag expression system. pHisC3, bacterial expression vector was designed. pHisC3 was used to generate expression vectors for PKC wild-type, catalytic domain and regulatory domain with a C-terminal 6×His-tag. These expression vectors are transformed into BL21(DE3) and then expressed by IPTG induction. Overexpressed protein was purified by Ni-column. Purified protiens will be assayed for kinase acivity. Then, they will be compared with expressed proteins in mammalian cell. We will develop novel substrates/binding proteins of PKCs using the purified PKCs. And we will study novel roles of individual isoforms of PKCs in cancer cell.

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