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제106회 대한화학회 학술발표회, 총회 및 기기전시회 안내 Application of Porous Silica Nanoparticles with Large Pores in Gene Delivery

2010년 8월 31일 15시 23분 43초
Ⅳ-MAT.P-159 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
금 <발표Ⅳ>
저자 및
김미희, 나희경, 김영관, 유수윤, 민달희
KAIST 화학과, Korea
Gene therapy using nanoparticle platform offers great promise for treatment of cancer and genetic disorders. The considerations of the nanoparticles to be used as drug carriers are ease of cellular uptake, gene expression efficiency, gene loading capacity, and biocompatibility. Silica nanoparticle has been attractive candidate as gene delivery vector because of several advantageous features such as facile synthesis and modification, stable porous structures, large surface areas, low cytotoxicity, and encapsulation of drugs and genes. In this report, we used expanded porous silica nanoparticles (EX-SiNP) with large pores for gene delivery system. This particle was aminated to hold pDNA through electrostatic interaction, and conjugated with TAMRA dye for imaging. Enough positive charges of particle according to the large surface area made possible to transfect into cells efficiently and have high loading capacity of pDNA. Moreover, in contrast to the conventional methods which require cationic lipids or polymers as supplementary materials, the present system doesn’t need to be supported by any polymers or lipids. We believe that this system contributes to the growth of the efficient drug delivery system and diagnostic tools due to considerable possibility of further applications such as siRNA delivery, large peptide/protein delivery, and highly sensitive biosensors.