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제107회 대한화학회 학술발표회, 총회 및 기기전시회 안내 Studies on the synthesis of α-Azidoether as a reversible terminator for DNA SBS (sequencing by synthesis)

등록일
2011년 2월 24일 09시 34분 27초
접수번호
1194
발표코드
Ⅳ-ORGN.P-270 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
발표시간
금 <발표Ⅳ>
발표형식
포스터
발표분야
유기화학
저자 및
공동저자
권태희, 이재균1, 배애님2, 이재열, 조용서2, 민선준2
경희대학교 화학과, Korea
1한국과학기술연구원 케모인포매틱스연구단, Korea
2한국과학기술연구원 생체과학연구본부, Korea
Over the past decades, DNA sequencing technology has become essential for various applied researches such as genomics, biotechnology, and biological systematics in addition to basic science area. Since the Sanger sequencing method dominated the industry, many next generation sequencing (NGS) methods have been developed for sequencing a large number of genomes with high efficiency and low cost. Recently, DNA sequencing by synthesis (SBS) approaches have been widely explored. The key feature of this method is that modified nucleotides attached to cleavable fluorophore moiety are required for repeating sequencing cycle. Herein, we report design and synthesis of new modified nucleotides having the reversible terminator at the 3′-OH position, which plays a dual role as a fluorescent signal reporter as well as a protective group. We are expecting that these new nucleotides replaced by α-azidoether at the 3′-OH are effectively introduced to DNA polymerase during polymerization. In addition, the fluorophore on the DNA extension products after determination of base will be easily removed under a mild reaction condition which is compatible with DNA.

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