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Electrochemical and Quartz crystal microbalance (QCM) studies on the interactions between carbohydrates and proteins

등록일
2005년 8월 11일 13시 54분 33초
접수번호
0870
발표코드
금15D4심 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
발표시간
금 11시 : 10분
발표형식
심포지엄
발표분야
전기화학 - 전기분석화학 심포지엄 (Analytical Elelctrochemistry Symposium)
저자 및
공동저자
류영구, 이원용
연세대학교 화학과,
Electrochemical and Quartz crystal microbalance (QCM) have been explored as alternatives to optical biosensors in order to study on the interactions between carbohydrates and proteins. Quartz crystal microbalance (QCM) has still inherent problems for the use of the piezoelectric mass sensing devices as biosensors, because the physics of biofilms in liquids are complex. This complexity makes it difficult to obtain an explicit relationship between the added mass and the change in the frequency output. In this work, thiol-modified carbohydrate immobilization approaches possibly overcome the above limitations for the use of the QCM with certain biofilms. A protein, concanavalian A (conA), from Canavalia ensilformis, which binds specifically to mannoside and glucoside, was used to study whether the frequency change was affected by nonspecific binding. The binding constants of the carbohydrates and proteins are measured by this approach. In addition, preliminary electrochemical method for this purpose will be discussed. QCM approach is significantly less expensive and more user-friendly and can be used to quantitate affinities in binding situations such as mannose and proteins.

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