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  • 03월 02일 17시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

제109회 대한화학회 학술발표회, 총회 및 기기전시회 안내 Development of LC/MS-based reference methods for food analysis: Combating matrix effects

2012년 2월 8일 18시 23분 17초
ANAL2-6 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
금 11시 : 50분
분석화학 - Recent Advances in Analytical Chemistry II
저자 및
한국표준과학연구원 삶의질측정표준본부, Korea
LC/MS is more frequently used than before in food analysis field due to the high selectivity and sensitivity of this technique. However, analytical methods based on LC/MS usually suffer from matrix effects (ion suppression/enhancement), and ignoring these factors may cause sometimes strong biases in measurement results. Adopting isotope dilution techniques (using isotope labeled analogues of target analytes as internal standards) has been believed to be the solution for matrix effects. Our laboratory is developing reference methods for food analysis which can provide national standards to ensure reliability of food testing laboratories. Those reference methods are based on Isotope Dilution Mass Spectrometry (IDMS) in connection with chromatographic techniques such as LC because IDMS is known to provide accurate analytical results without systematic bias through the accurate correction of the recovery on target analytes along the sample clean up processes. However, we observed in many cases that matrix effects are not completely compensated when deutrated analogues are used as they have slightly different chromatographic retention times from their corresponding target analytes and experience different ionization conditions. A few examples of those cases and solutions for these adverse effects will be presented. Solutions adopted were more rigorous sample clean-up and optimizing chromatographic separation. Recently, we adopted a standard addition method in combination with isotope dilution techniques for alternative solution when those strategies do not work