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  • 03월 02일 17시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

제109회 대한화학회 학술발표회, 총회 및 기기전시회 Home-built Solid-state NMR Probe with Specialized Coil

2012년 2월 15일 10시 31분 04초
ANAL.P-552 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
4월 25일 (수요일) 18:00~21:00
저자 및
최성섭, 정지호, 박유근, 김용애
한국외국어대학교 화학과, Korea
Analysis of membrane protein using solid-state NMR on lipid bilayer or bicelle samples, membrane-like environment, is valuable for these with a predominantly helical secondary structure. Most of biological lipid samples have high dielectric property due to containing large amounts of water and salts. This electrical property cause a loss of probe efficiency from severely reducing the probe Q-factor and significantly shifting the tuned frequency down. For these reasons, a specific probe with high efficiency and high capability is required to study these biological samples by using solid-state NMR, and several probe designs have been developed in a recent research to improve the probe efficiency. Here, we present the optimized design, construction, and efficiency of a home-built 400 MHz wide-bore 1H-15N solid-state NMR probe with 5-mm solenoidal rf coil and a home-built 800 MHz narrow-bore 1H-15N solid-state NMR probe with strip-shield coil to prevent heating by high RF power. This probe provides short pulses, high power capability, and good RF homogeneity. 1H-15N 2D SAMMY spectra from a single crystal and membrane proteins in oriented phospholipid bicelles was successfully obtained by using this home-built solid-state NMR probe. Inspired by the natural and technical similarities between membrane proteins in lipid bilayers and liquid crystals in Liquid Crystalline Display (LCD) panels, we employed solid-state NMR methodologies originally developed for the study of membrane proteins in lipid bilayers for the in-situ analysis of LCD panels. Thus, we also present a home-built 500 MHz narrow bore (NB) 19F-13C double resonance solid-state NMR probe with a flat-square coil and the first application of this probe for the in-situ analysis of LCD panel samples.