Carpinus turczaniowii is a tree belonging to Betulaceae family. Phytochemical investigation of the ethanol extract from branches of C. turczaniowii by using repeated column chromatography resulted in the isolation of eight compounds; β-sitosterol (1), daucosterol (2), betulinic acid (3), pyracrenic acid (4), carpinontriol B (5), carpinontriol A (6), afzelin (7) and quercitrin (8). The structures of these compounds were identified by spectroscopic methods and by comparing their data to those in the literature. As far as we know, all of the compounds except quercitrin (8) were isolated for the first time from this plant.
The isolated compounds were also subjected to the activity screenigs on anti-oxidation and anti-inflammation. For the anti-oxidation tests, the compounds 4 and 8 showed strong DPPH radical scavenging activities with SC50 of 55.2 and 62.4 μM respectively, whose activities were comparable to a positive control vitamin C (SC50 43.5 μM). In addition, compounds 4, 5, 6 and 8 showed strong ABTS+ radical scavenging activities with SC50 of 34.1, 42.1 45.8 and 29.6 μM respectively, indicating comparable activity to vitamin C (SC50 31.6 μM).
On the screening of anti-inflammatory activities, the compounds 5 and 6 showed the considerable inhibition on the production of nitric oxide for the RAW 264.7 cell without exhibiting cell toxicities.
Based on these results, C. turczaninowii branch extract could be potentially applicable as anti-oxidant and anti-inflammatory agents in cosmeceutical and/or pharmaceutical preparations.