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  • 03월 02일 17시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

제109회 대한화학회 학술발표회, 총회 및 기기전시회 안내 Screening of ssDNA aptamers using Cell-SELEX against Salmonella typhymurium

2012년 2월 16일 15시 18분 56초
BIO.P-680 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
4월 25일 (수요일) 18:00~21:00
저자 및
이상춘, 윤문영
한양대학교 화학과, Korea
Food poisoning typically caused by pathogenic bacteria and their accumulated/released toxins. In the review of literature, the Salmonella spp. and Escherichia spp. are the major/wide spread pathogenic bacteria found the cause for food poisoning in Korea. The emergence of rapid diagnostic systems to detect these pathogenic bacteria was attracted much attention due to the findings of increased food poisoning occurrence from the imported food and public restaurants. Aptamers, generated from Systematic Evolution of Ligands by Exponential Enrichment (SELEX) are promising as diagnostic probes because of their high binding affinity against wide range of targets. Several SELEX methods were reported to date, generally depend on the targets used. Cell-SELEX is one among which has several advantages on aptamer selection. In this study, we performed Cell-SELEX to identify ssDNA aptamers against S. typhymurium. The stringent conditions including, salt concentrations, cell number and ssDNA pool concentrations were applied as Cell-SELEX rounds progress to obtain high affinity/specific binding aptamers. After 10 rounds of Cell-SELEX the pools were cloned and sequenced. A total of 10 aptamer sequences were generated from the 10th round product of Cell-SELEX. Finally, 3 aptamers against S. typhymurium which binds with high affinity were synthesized with fluorescence labeling as a signal probe. Each aptamer binding affinity and specificity were characterized, respectively. Further studies of modification and conjugation of aptamers with nano-materials would expect the development of a sensitive detection system against S. typhymurium.