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  • 03월 02일 17시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

제109회 대한화학회 학술발표회, 총회 및 기기전시회 안내 Synthesis of a two-photon absorbing compound toward reactive fluorescent probes : An application to probing monoamine oxidase activity

2012년 2월 16일 15시 30분 58초
ORGN.P-886 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
4월 25일 (수요일) 18:00~21:00
저자 및
김도경, 안교한
포항공과대학교 화학과, Korea

Monoamine oxidases (MAOs) play an important role in regulating tissue levels of amine neurotransmitters and dietary amines. Much efforts have been made to search for MAOs inhibitors, which have potential for the treatment of neurological disorders such as Parkinson`s disease and schizophrenia. It is thus important to develop homogeneous and highly sensitive assay systems for MAOs’ activity, which may be used for drug discovery and for monitoring enzymatic activities in complex biological systems. The known fluorescent probes for MAOs are based on one-photon fluorophores, for example, resorufin and quinazolinone derivatives. Two-photon probes for MAOs, however, remain unexplored, in spite of their advantageous features over the one-photon probes. We have developed a novel type of fluorescent probes, with which MAOs’ activity can be assayed in vitro and imaged in cells by both one- and two-photon microscopy. Coumarin precursors (the probes) thus undergo a MAO-catalyzed oxidation reaction followed by hydrolysis to afford a β-aryloxyaldehyde intermediate, which further undergoes β-elimination followed by an intramolecular cyclization to afford an imino-coumarin product (Imino-POS) that emits fluorescence in the turn-on mode, either by one-photon or by two-photon excitation