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제109회 대한화학회 학술발표회, 총회 및 기기전시회 안내 Comprehensive analysis of lipid raft proteome of MCF-7 and MCF-7/ADR cells

등록일
2012년 3월 2일 14시 21분 39초
접수번호
1565
발표코드
ANAL.P-648 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
발표시간
4월 25일 (수요일) 18:00~21:00
발표형식
포스터
발표분야
분석화학
저자 및
공동저자
문동기, 김수진, 이상원
고려대학교 화학과, Korea
There have been reports that detergent-resistant lipid rafts play important roles in multidrug resistance (MDR). In this work, comprehensive analysis of lipid rafts proteome isolated from MCF-7 and MCF-7/ADR cells are described. Ultra-high pressure microcapillary liquid chromatography/tandem mass spectrometry based approaches were applied identifying 936 proteins in lipid raft proteomes. High throughput protein quantitation was facilitated by label-free quantitative analysis, discovering 176 differentially expressed proteins with p-value of less than 0.05. Comparison of quantity of peptide feature was done by introducing unique mass class (UMC). Among 176 proteins, polymerase transcription release factor (PTRF)/cavin-1 was observed to be upregulated along with multidrug-resistant P-glycoprotein (P-gp), caveolin-1 and serum deprivation protein response (SDPR)/cavin-2 in the lipid rafts of MCF-7/ADR cells. Extracted ion chromatogram as well as results of RT-PCR and immunoblotting supports their highly upregulation in MCF-7/ADR cell. Subsequent experiment such as measuring fluidity of PTRF knockdown cells indicates the necessity of PTRF for the regulation of MDR in MCF-7/ADR cells.

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