초록문의 abstract@kcsnet.or.kr

결제문의 member@kcsnet.or.kr

현재 가능한 작업은 아래와 같습니다.
  • 09월 12일 17시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

제110회 대한화학회 학술발표회, 총회 및 기기전시회 안내 Analysis of synthetic oligonucleotides arrays using Microscopic MALDI Imaging Mass Spectrometry

2012년 9월 5일 10시 14분 21초
ANAL.P-702 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
10월 17일 (수요일) 16:00~19:00
저자 및
오주연, 윤소희1, 이태걸1
한국표준과학연구원 나노바이오융합연구단, Korea
1한국표준과학연구원 미래융합기술부, Korea
Imaging mass spectrometry has become an advanced methodology for its capability of simultaneous detection of all desorbed analytes over a sample surface. Imaging mass spectrometer (IMS) coupled with matrix-assisted laser desorption/ionization (MALDI) and time of flight (TOF) makes possible macromolecules analysis with high sensitivity., An ion-optical microscopic lens system was designed and constructed to project the spatial origin of the desorbed ions from the sample surface onto the position sensitive detector(IONPIX, AMOLF, The Netherlands), for a microscopic MALDI IMS. A spatial resolution is determined by ionizing beam size, the quality of ion optics, magnification of microscope system, and the detector resolution. The spatial resolution down below 10 um can be achieved for a large field of view of 500 x 500 um2 in preliminary experiments. The deposition of more spots in a field of view, means that more samples can be analyzed at a time. It results in reducing the time for analysis. This methodological approach is able to produce high throughput data with acquisition times reduced by more than three orders of magnitude compared with microprobe imaging technique. Its application to the high-throughput DNA analysis is also demonstrated.