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학술발표회초록보기

초록문의 abstract@kcsnet.or.kr

결제문의 member@kcsnet.or.kr

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  • 09월 12일 17시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

제110회 대한화학회 학술발표회, 총회 및 기기전시회 안내 Development of Image-Based High-Throughput Screening for Monitoring Glucose Uptake in Live Cells

등록일
2012년 9월 5일 15시 53분 24초
접수번호
1716
발표코드
BIO.P-780 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
발표시간
10월 17일 (수요일) 16:00~19:00
발표형식
포스터
발표분야
생명화학
저자 및
공동저자
조아라, 박종민
서울대학교 화학부, Korea

We previously developed novel fluorescent glucose analogue GB which is applicable to fluorescence-image based, real-time and quantitative monitoring of glucose uptake in living cells. Because of its instinsic physological chacter that it does not serve as substrate for hexokinase, however, we consistently observed substantial release of GB2 from the cells. To avoid this potential problem for its application to large-scale compounds screening, we introduced inhibitor of glucose transporter ‘phloretin’ in glucose uptake monitoring assay. From this improvement, we developed rapid and reliable high-throughput screening assay for glucose uptake in living cell. On the basis of the importance of glucose homeosis in human, image-based HTS system for monitoring glucose uptake in live cell would be a powerful tool in the discovery of therapeutic agents for related disease. Furthermore, image-based assay at cellular level allows dual tracing of other cellular aspects and glucose utilization, providing intensive screening system and potential to discover undiscovered relation between glucose utilization and novel cellular process.


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