We have developed a highly active enzyme by coating Burkholderia cepacia lipase with an ionic surfactant for use in DKR. With this ionic-surfactant-coated Burkholderia cepacia lipase (ISCBCL), we were able to achieve the highly enantioselective DKR of a wide range of secondary alcohols previously unexplored. Different secondary alcohols were tested as the substrate of ISCBCL to see its substrate scope and enantioselectivity in KR and DKR . In most cases, good yields and high enantiomeric excesses were obtained1,2
This work was supported by the National Research Foundation of Korea (KRF-2007-511-C00031 and 2009-0074357)
1. Reviews: (a) J. H. Lee, K. Han, M.-J. Kim, J. Park, Eur. J. Org. Chem. 2010, 999-1192. (b) Y. Kim, J. Park, M.J. Kim, ChemCatChem, 2011, 3, 271-277.
2. H. Kim, Y. K. Choi, J. Lee, E. Lee, J. Park, M-J. Kim, Angew. Chem. Int. Ed. 2011, 50, 10944