KCS

We fabricated doxorubicin (DOX) or other mycotoxin-assembled beta-cyclodextrin thiolate (beta-CD-SH) on gold nanoparticles (AuNPs). The inclusion of DOX to beta-CD-SH and their subsequent adsorption on the AuNP surfaces was characterized using Raman, UV-Vis absorption, quasi-static light scattering, and infrared spectroscopy. A micromolar range of DOX loading is estimated for either 5 nm or 18.5 nm AuNP particle platform covered by beta-CD-SH. The fluorescence recovery test indicated that the attached DOX released from AuNPs after treatment with not serum media but 2 mM glutathione (GSH). Fluorescence microscope imaging indicated that the DOX was released from AuNPs in the cervical cancer HeLa cells after the endocytosis. We performed a cell viability assay for the anticancer drug-AuNP conjugates in the delivery to HeLa cells. A cytotoxicity test indicated that the DOX/beta-CD-SH/AuNPs system reduced the cell viability, whereas a comparable amount of AuNPs did not show any noticeable cytotoxicity. Our characterization may be useful to design the anticancer therapy based on AuNPs and CDs. We plan to develop a new spectroscopic detection method of mycotoxins using these platforms.