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  • 02월 28일 19시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

제111회 대한화학회 학술발표회, 총회 및 기기전시회 안내 Generation and characterization of engineered split-inteins

등록일
2013년 2월 14일 16시 36분 18초
접수번호
1222
발표코드
BIO.P-607 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
발표시간
4월 17일 (수요일) 16:00~19:00
발표형식
포스터
발표분야
생명화학
저자 및
공동저자
전용권, 정덕호, 권영은*
동국대학교 의생명공학과, Korea
Protein trans-splicing reaction (PTS) is self-processing enzymatic reaction mediated by a pair of split-inteins. PTS based protein semi-synthesis enables the introduction of various synthetic probes to target proteins in vivo and in vitro. While the PTS became a useful tool for various biological studies, the difficulties associated with the synthesis of 40mer peptide became a drawback in its applications. Here we have generated several engineered split-inteins by shifting the split-site of naturally split-inteins to new positions. We utilized Npu DnaE intein as a model system as this naturally split-intein mediates fast (t1/2 ~ 1 min) PTS in physiological conditions. In order to investigate how the lengths of N and C-intein affect the PTS kinetics, we prepared multiple pairs of split-inteins that consist of various lengths of N and C-inteins. The kinetics of reaction between each engineered pairs and the cross-reactivity were studied.

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