abs

학술발표회초록보기

초록문의 abstract@kcsnet.or.kr

결제문의 member@kcsnet.or.kr

현재 가능한 작업은 아래와 같습니다.
  • 02월 28일 19시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

제111회 대한화학회 학술발표회, 총회 및 기기전시회 안내 Rapid monitoring of CFP-10 during culture of Mycobacterium tuberculosis by using a magnetophoretic immunoassay

등록일
2013년 3월 4일 09시 41분 31초
접수번호
1487
발표코드
ANAL1-6 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
발표시간
목 11시 : 20분
발표형식
심포지엄
발표분야
분석화학 - Recent Advances in Inorganic Material Analysis
저자 및
공동저자
이재범
부산대학교 나노메디컬공학과, Korea
We introduce a rapid and robust sensing kit using sandwich-type magnetophoretic immunoassay (MPI) for the colorimetric detection of a secreted tuberculosis (TB)-associated antigen, CFP-10, in cultures of Mycobacterium tuberculosis (Mtb). Detection using this assay relies on the antigen-specific formation of complexes between Au nanoparticles (NPs) and magnetic microparticles (MMPs), such that magnetophoresis in the presence of CFP-10 decreases the plasmon absorbance of the assay solution due to the removal of Au NPs. The MPI offers rapid (results are obtained in 10 min), quantitative, and qualitative monitoring of CFP-10 expression in a detection range from 10 to 10,000 pg/mL with a detection limit of 10 pg/mL. Assay validation was performed by monitoring CFP-10 secreted during the Mtb culture. The data revealed a linear relationship between the concentration of CFP-10 in culture supernatants and the duration of Mtb culture. Thus, it shows high potential that the rapid and robust MPI sensing kit can be used for early TB diagnosis through immunoassay detection of TB-specific antigens in Mtb culture with further clinical investigation.

상단으로