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제112회 대한화학회 학술발표회, 총회 및 기기전시회 안내 Solvatochromic properties of coumarin dyes and their applications to amyloid aggregation probing

등록일
2013년 8월 26일 14시 16분 53초
접수번호
0216
발표코드
PHYS.P-277 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
발표시간
10월 16일 (수요일) 16:00~19:00
발표형식
포스터
발표분야
물리화학
저자 및
공동저자
이휘인, 이민영*
이화여자대학교 화학나노과학과, Korea
Solavatochromic fluorescence dyes such as ANS and bis-ANS have been widely used to probe protein aggregation. We explored two coumarin dyes, coumarin 6 (C6) and coumarin 7 (C7), as new solvatochrmic dyes and used C6 and C7 to investigate the early stage of amyloid aggregation processes. The emission spectra of both dyes showed a significant spectral shift as bound to amyloid oligomers in comparison with the monomeric state in buffer. The sizes of amyloid oligomers were characterized by high resolution AFM as a function of incubation time. The steady-state and time-resolved fluorescence were employed to monitor the binding affinity of C6 and C7 to amyloid aggregates. The binding mode and association constant of the coumarin dyes to amyloid were analyzed on the basis of the molecular structure of oligomer aggregates.

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