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제112회 대한화학회 학술발표회, 총회 및 기기전시회 안내 Separation Analysis Technology Using Supercritical Fluid Chromatography

2013년 8월 29일 13시 34분 52초
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목 11시 : 35분
분석화학 - KCS-JASIS Joint Symposium: Advanced Separation Science
저자 및
Yoshiteru Horikawa
JASCO Corporation, Japan
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승인 1건
Supercritical Fluid Chromatography (SFC) offers high speed analysis due to high diffusivity of solutes in the low-viscosity supercritical mobile phase. Generally, the separation mode in SFC is regarded as normal phase with a combination of non-polar CO2 mobile phase and polar stationary phase. Therefore, separation of water-soluble polar compounds is considered to be difficult to separate by using SFC. However, the elution and peak shapes of polar compounds can be improved by adding a small amount of acid, base, volatile salt and ion pair reagent to the modifier solvent such as alcohol. Supercritical carbon dioxide evaporates when it is released in the air. This takes the advantage of easy solvent removing for fractionation. SFC has become popular for separation and fractionation of chiral substances. The screening method, which comprehensively analyzes the interests by the combinations of various solvents and columns, has been used to achieve a suitable separation of chiral substances. We demonstrate the rapid screening of several chiral medicines. It took only 6 hours to search a suitable separation. In chiral preparative SFC, samples are often over-loaded and it is difficult to determine when to start and end fractionation from Ultra Violet (UV) chromatographic data. A Circular dichroism (CD) detector can differentiates enantiomers and generate positive/negative chromatographic peaks. In addition, CD detector can simultaneously generate CD, UV and g-factor signals. The g-factor is a signal defined as CD/UV whose level is independent of the peak concentration but dependent only on enantiopurity. Therefore, enantiopurity of g-factor triggered fractions is very high.