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제112회 대한화학회 학술발표회, 총회 및 기기전시회 안내 A Novel Homogeneous Assay for Simultaneous Determination of Fluoroquinolones in Water based on Upconversion Fluorescence Resonance Energy Transfer

2013년 8월 29일 17시 00분 14초
ANAL.P-522 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
10월 16일 (수요일) 16:00~19:00
저자 및
GerelkhuuZayakhuu, 강지훈, 이용일*, Zhen Zhang1
창원대학교 화학과, Korea
1school of the environment ,Jiangsu University, China
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승인 1건
A novel homogeneous method for simultaneous sensing of trace fluoroquinolones (FQs) in water using upconversion fluorescence resonance energy transfer (FRET) was developed. Lanthanide-based upconversion nanophosphors(UCPs) have attracted much attention because of wide application in biological labeling, sensing, and imaging.It was attributed to its unique characteristics of low toxicity, nearly-zero background auto-fluorescence, narrow emission band, high photoluminescence intensity, and photostability. Although the surface functionalization of UCPs with good hydrophilic and strong luminescence inaqueous solution has been performed, the application in biological assays is still few, especially for organic pollutants in foodstuffs and environmental waters. We present the facile synthesis of monodisperse, size-tunable,and highly efficient UCPs(β-NaLuF4:Gd,Yb,Er3+) linked with -COOH groups which can be used as an donor after conjugation with our produced FQs monoclonal antibody. The corresponding antigens labeled gold nanoparticles (AuNPs) was treated as an acceptor. In competitive reaction, dissolved FQs and labeled AuNPs competed each other to combine with FQs antibodies labeled UCNPs. The original green light was quenched by the acceptorwhen exited by Near-infrared light (980 nm) to donor, and the intensity shows the linear correlationship with the concentration of FQs in water. Keywords: Upconversion nanoparticles, fluoroquinolones, fluorescence resonance energy transfer (FRET), near-infrared light