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제112회 대한화학회 학술발표회, 총회 및 기기전시회 안내 Comparison of isotope patterns of hemin and standard peptide ions in Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

등록일
2013년 9월 5일 11시 29분 06초
접수번호
1357
발표코드
ANAL.P-534 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
발표시간
10월 16일 (수요일) 16:00~19:00
발표형식
포스터
발표분야
분석화학
저자 및
공동저자
김태희, 이지현, 김정권*
충남대학교 화학과, Korea
In this study, we investigated the change of isotope pattern of heme ion from myoglobin and cytochrome c upon the change of the irradiated laser power in the matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). When the laser intensity was increased (from 1519 to 2519 with DHB matrix and from 1087 to 2187 with CHCA matrix), the ratio of isotope distributions of heme c was generally increased. The theoretical relative abundance of the second isotope compared to the first isotope of heme c was 40.96%, but experimental relative abundance was increased from 53.48% to 88.56% with DHB matrix and from 42.76% to 97.54% with CHCA matrix. However the ratio of isotope distributions of heme b was not changed. The theoretical relative abundance of the second isotope of heme b was 40.95%, and experimental relative abundance was about 75.71% with DHB matrix and was about 55.24% with CHCA matrix. In this study, we compared the isotope patterns of hemin and standard peptide ion upon the change of laser intensity in MALDI-TOF MS.

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