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학술발표회초록보기

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  • 09월 06일 15시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

제112회 대한화학회 학술발표회, 총회 및 기기전시회 안내 Synthesis and evaluation of an engineered molecule as a tool for the proteomic study

등록일
2013년 9월 5일 14시 49분 06초
접수번호
1382
발표코드
BIO.P-602 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
발표시간
10월 16일 (수요일) 16:00~19:00
발표형식
포스터
발표분야
생명화학
저자 및
공동저자
김은주*, 강동욱1,*
대구대학교 과학교육학부/화학교육과, Korea
1대구가톨릭대학교 제약산업공학과, Korea
A novel substance has been designed, synthesized, and evaluated as a tool for the proteomic study. This new molecule consists of a biotin moiety at the one end and an azide-reactive chemical tag at the other end with a cleavable linker placed between these two entities. Biotin molecule is one of the most popular epitope tags for the detection and enrichment of molecules of interest, because of its strong interaction with streptavidin. However, their strong binding also exerts a negative effect when the bound biotinylated molecules are eluted from the streptavidin solid support. Harsh conditions required for interrupting the strong biotin:streptavidin interaction to release the bound molecules from the solid support, can lead to decomposition of the molecules, resulting in low product recovery. In order to tackle this problem, an engineered molecule, wherein a disulfide linker is inserted in the middle of the molecule to connect a biotin tag and an azide-reactive chemical tag, has been synthesized and examined for its utility as a tool in the proteomic study.

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