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  • 09월 06일 15시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

제112회 대한화학회 학술발표회, 총회 및 기기전시회 안내 Radio immunoassay of [123I]iodoemodin for HER-2 receptor as inhibitor

2013년 9월 5일 16시 06분 28초
MEDI.P-958 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
10월 16일 (수요일) 16:00~19:00
저자 및
박정훈, 이준영1, 허민구, 양승대, 김상욱1, 유국현2,*
한국원자력연구원 방사선기기연구부, Korea
1동국대학교 신소재화학과, Korea
2동국대학교 화학과, Korea
The aim of this study is synthesis and evaluation the radio labelled emodin as radiopharmaceutical for cancer cell diagnosis and therapy. The labeling of 123I was performed in [123I]NaI solution (1110 GBq/ 100 μL). 0.5 M H3PO4, 32% peracetic acid with emodin solution in methanol (3 mg/mL) then stirring for 10 minutes. The compound was perified by RP-HPLC. Cell uptake experiments Both of the breast cancer cells MCF-7 and SK-BR-3 cell lines were seeded at in 24 well plates at 1 mL per well and incubated at 37 ℃ in a 5% CO2 for 24 h for an adherence and growth. To each well in 2-[123I]iodoemodin (185 KBq) was added. Cells were washed twice in cold phosphate buffered saline (PBS) at designated time point (10 min, 30 min, 60 min and 120 min). The cell pellets were counted by gamma counter. Data was presented as a percentage of the injected radioactivity dose (%ID). The synthesis of Iodoemodin was conformed by 1H-NMR spectroscopy. Iodone was found to be introduced at the 2-positron of the emodin from the disappearance of the proton signal of the 1H NMR spectrum at 6.6 ppm. Purification of [123I]iodoemodin was carried out by a RP-HPLC using ammonium acetate and methanol. As a result, the peak was found about 25 minutes. The cellular uptakes of 2-[123I]iodoemodin increased in a time dependent manner for both breast cancer cell lines (SK-BR-3 and MCF7). We have synthesized and evaluated 2-[123I]iodoemodin on human breast cancer cells (SK-BR-3 and MCF-7) which express of HER-2 receptor. The results of this study can be used as a breast cancer imaging reagent for PET