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  • 09월 06일 15시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

제112회 대한화학회 학술발표회, 총회 및 기기전시회 안내 Detection of Anti-Neospora Antibodies in Bovine Serum by Au-CdTe Nanocomplex

2013년 9월 5일 16시 28분 41초
ANAL.P-535 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
10월 16일 (수요일) 16:00~19:00
저자 및
주굉건, 이재범1,*
부산대학교 나노융합기술대학, Korea
1부산대학교 나노메디컬공학과, Korea

Detection of biomolecules via nanotechnology has become increasingly important in a variety of fields, including medical diagnostics, food safety, and anti-bioterrorism. Neosporosis is an infectious disease primarily of cattle, which is caused by an intracellular parasite, Neospora caninum, and now appears to a major cause of abortion in dairy cattle worldwide. Therefore, we report a rapid, sensitive, and inexpensive qualitative approach for neosporosis detection based on the photoluminescence (PL) enhancement between quantum dots (CdTe nanoparticles) and a unique shape of Au nanoparticles, called Au nanoparticles (UNPs) conjugated by antibody-antigen. Anti-bovine IgG functionalized-UNPs were then bound to complementary target, anti-Neospora antibodies (ANABs) in bovine serum. The binding of analyte to the molecular probe was monitored by the PL enhancement of CdTe nanoparticles in the PL spectrum resulting from a combination with surface roughness of Au nanoparticles by the immunological reaction. The fluorescence intensity was compared in the samples of infected and healthy cattle, resulting in significant intensity difference. The Au-QDs sandwich nanocomplex remains in solution and its optical properties allow it to be easily quantified using fluorescence absorption spectroscopy. More than 55% emission enhancement on the surface of the urchin-like NPs compared with those of CdTe NPs was observed, and the results exhibit good reproducibility. Further, the biosensor was suited for qualitative analysis of ANABs in the actual media of blood serum. The ease of operation, high sensitivity, and its generality offer specific advantages over other immunoassay methods.