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  • 09월 06일 11시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

제112회 대한화학회 학술발표회, 총회 및 기기전시회 안내 Glycoproteome Profiling of Human Gastric Cancer Tissue Using FACE (Filter Aided Capture and Elution) and UPLC-MS/MS

2013년 9월 5일 16시 42분 28초
BIO1-3 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
목 09시 : 50분
생명화학 - Mass Spectrometry for Biological Applications
저자 및
이후근*, 박종문1
가천대학교 메디컬캠퍼스 약학대학, 이길여 암당뇨연구원, Korea
1가천대학교 메디컬캠퍼스 이길여암당뇨연구원, Korea
Protein glycosylation is the most important post-translational modifications (PTMs). Glycoproteins are often important integral proteins that play a role in cell-cell interactions,, developmental biology, cell signaling and inflammation in cell membrane. Because of low stoichiometry of modified proteins, for identifying the numerous glycoproteins, the proteomics analysis of these PTM requires powerful and indispensable technology. To detect low abundant glyco-peptides or proteins in complex mixture among the high abundant non glycopeptide equivalents with LC-MS technologies, enrichment methods have to be applied. For example hydrazide bead, ERLIC (Electrostatic Repulsion Hydrophilic Interaction Chromatography), HILIC (Hydrophilic Interaction liquid Chromatography), lectin affinity chromatography method have widely been used for enrich glycol- peptides or proteins. In this study, we introduce a powerful protocol for enrichment of glycopeptides, and analyzed glycosylation of human gastric cancer tissue using membrane based modified FACE (filter aided capture and elution) method. So we were identified 3,000 N-glycosylated peptide. And then, we functional studies of the normal samples compared with cancer sample using IPA (Ingenuity Pathway Analysis) from the list of identified glycopeptides.