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  • 09월 06일 15시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

제112회 대한화학회 학술발표회, 총회 및 기기전시회 안내 Single Molecule Optical Analysis of Various Yeast Mitochondria DNA Sizes

등록일
2013년 9월 5일 16시 43분 37초
접수번호
1441
발표코드
BIO.P-604 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
발표시간
10월 16일 (수요일) 16:00~19:00
발표형식
포스터
발표분야
생명화학
저자 및
공동저자
최소정, 김용균, 이진용, 조규봉*
서강대학교 화학과, Korea
Saccharomyces cerevisiae is a yeast strain used for making wine and bread. Since it is a model organism for the study of eucaryotes, its genome has been fully sequenced that there are 16 chromosomes and a mitochondrial genome. However, a couple of previous studies reported that they found more sequences from mitochondrial DNA that was not previously sequenced. In addition, some other studies showed that mitochondrial DNA did not have the same size by using single DNA molecule experiment. Thus far, it is not clear how mitochondrial DNAs are different from each other in a yeast cell. Here we present their difference by using single molecule DNA analysis such as Optical Mapping, in which whole mitochondrial genomic DNAs are elongated and immobilized on the positively charged surfaces within a microfluidic device and digested by restriction enzyme such as EagI. Restriction enzyme digested barcode-like DNA patterns provides the information how mitochondrial genomic DNAs are different from each other.

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