abs

학술발표회초록보기

초록문의 abstract@kcsnet.or.kr

결제문의 member@kcsnet.or.kr

현재 가능한 작업은 아래와 같습니다.
  • 02월 20일 17시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

제113회 대한화학회 학술발표회, 총회 및 기기전시회 안내 A Ratiometric Two-Photon Probe for Real-Time Imaging of Lysosomal pH

등록일
2014년 2월 13일 16시 05분 02초
접수번호
1064
발표코드
ORGN.P-771 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
발표시간
4월 16일 (수요일) 16:00~19:00
발표형식
포스터
발표분야
유기화학
저자 및
공동저자
김형중, 허철호, 김환명*
아주대학교 에너지시스템학부, Korea
Acidic pH plays crucial roles in many aspects of cell metabolism. To extend our understanding of the roles of acidic environments in biology and pathology, it is essential to monitor subcellular pH values at the cell, tissue, and organism level. However, most conventional probes have limitations imposed by fluorescent turn-on responses at single detection window and/or their short excitation wavelength encountered. An attractive approach to the detection of pH value in live cells and tissues is ratiometric imaging with two-photon microscopy (TPM). TPM, which employs two near-infrared photons as the excitation source, offers a number of advantages including greater penetration depth (> 500 μm), localization of excitation, and longer observation times. We report small molecule and ratiometric TP probes (BH1-3 and BH1L) derived from benzimidazole for monitoring acidic pH in subcellular compartments. These probes have a bright two-photon excited fluorescence, a marked blue-to-green emission color change in response to pH 7.5-4.0 with pKa values of 4.9-6.1, a distinctive isoemissive point, negligible cytotoxicity, and high photostability, thereby allowing real-time quantitative imaging of intracellular pH.

상단으로