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제113회 대한화학회 학술발표회, 총회 및 기기전시회 안내 A proline-derived two-photon fluorescent probe for intracellular sodium ions

2014년 2월 13일 16시 05분 19초
ORGN.P-772 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
4월 16일 (수요일) 16:00~19:00
저자 및
Sarkar Avik Ranjan, 이효원1, 김환명1,*
아주대학교 나노정보융합기술연구소, Korea
1아주대학교 에너지시스템학부, Korea
The design of a synthetic probe to sense sodium ions is of extreme importance in the biomedical field because it plays vital roles in many physiological and pathological processes. In the detection of Na+, a handful one-photon (OP) fluorescent probes have been reported in literatures. The main drawback of these probes is that they are bulky and suffer from difficulties in loading into live cells. The second one is their short excitation wavelength (<500 nm). This requirement limits their application in tissue imaging owing to the shallow penetration depth (<100 μm), photobleaching, and cellular auto-fluorescence. An attractive approach to overcome these problems and understand the role of Na+ in biological processes is the use of two-photon microscopy (TPM), which utilizes two near-infrared photons of lower energy for the excitation. Previously, we reported Na+ ion selective TP probe (ANa1) that has been selectively detect Na+ in live cell and tissue by TPM. Based on promising properties of ANa1, herein, we report an efficient turn on TP probe (ANa2) that shows a maximum TP action cross-section of 80 GM with a dissociation constant (KdTP) value of 22 mM and strong TPEF enhancement in response to Na+. This probe can selectively detect intracellular free Na+ in live cells and living tissues without interference from other metal ions.