Two-photon (excitation) microscopy (TPM) combined with the laser excitation technology has arrested recent attention in the bioimaging area because it shows several advantageous features over one-photon excitation microscopy (OPM). TPM is most often emphasized to be “less photo-toxic, less photo-bleaching and less photo-damaging”. In addition, TPM enables deeper tissue (700-1100 nm) imaging with high spatio-temporal resolution, and thus is particularly useful for tissue imaging where auto-fluorescence becomes serious in the case of OPM.
The primary aim of this research is to develop new two-photon materials which show desirable photo-physical properties for bioimaging, overcoming some drawbacks of known dyes such as acedan and coumarin derivatives. This research is also focused on the studies of new platforms for the development of two-photon fluorescent probes for biologically important analytes, based on the reaction-based sensing approach. As a results, we have developed novel π-extended coumarin dyes, which show very promising properties as two-photon excitable dyes and also molecular shape-dependent luminescent behaviours.
#Ref: (a) Kim, Kim, D.; Sambasivan. S.; Nam. H.; Kim. K. H.; Kim. J. Y.; Joo. T.; Lee. K.; Kim. K.; Ahn. K. H. Chem. Commun. 2012, 48, 6833. (b) Kim, I,; Kim, D.; Ahn. K. H. Asian J. Org. Chem. 2012, 1, 60.