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  • 02월 20일 17시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

제113회 대한화학회 학술발표회, 총회 및 기기전시회 안내 Dopamine and Cu+/2+ can induce oligomerization of α-synuclein in the absence of oxygen: two types of related oligomerization mechanisms for α-synuclein

2014년 2월 18일 11시 08분 17초
BIO.P-571 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
4월 16일 (수요일) 16:00~19:00
저자 및
하용황, D.G.Churchill*
한국과학기술원(KAIST) 화학과, Korea
It has been hypothesized and supported that α-synuclein oligomer can induce the toxicity in Parkinson’s disease progression. Although many studies reported α-Syn oligomerization by dopamine (DA) and transition metal ions, there are few studies about joint influences of DA and Cu2+. In this study, DA and Cu2+ were co-treated for measuring of α-Syn oligomerization. In the presence of oxygen, DA induced α-Syn oligomerization in a dose-dependent manner. Cu2+ did not effect oligomerization in a dose-dependent manner in the presence of DA. By electrophoresis, Cu2+ ions are shown to change oligomers which were induced by DA into other types easily. This implies oligomerization reaction by DA is reversible in the presence of Cu2+. In the absence of oxygen, DA induced less oligomerization of α?synuclein whereas DA/Cu2+ induced aerobic?level amounts of oligomers. This suggests that DA/Cu2+ can induce oligomerization independent of oxygen. Radical species were detected through EPR analysis, from co?incubation of α?synuclein, DA and Cu2+. Redox reactions induced by DA/Cu2+ were observed in multimer regions of α-synuclein oligomers through NBT assay. From these results, we discussed DA and DA/Cu2+ induce different mechanisms on α-synuclein oligomerization: cross?linking versus radical?mediated covalent modification.