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학술발표회초록보기

초록문의 abstract@kcsnet.or.kr

결제문의 member@kcsnet.or.kr

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  • 02월 20일 17시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

제113회 대한화학회 학술발표회, 총회 및 기기전시회 안내 FITGE-Based Target Identification for the Connection of Rational Drug Discovery with Phenotypic Screening

등록일
2014년 2월 18일 16시 04분 32초
접수번호
1265
발표코드
ORGN2-2 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
발표시간
목 14시 : 00분
발표형식
심포지엄
발표분야
유기화학 - Current Trends in Organic Chemistry II: Functional Organic Materials
저자 및
공동저자
박승범
서울대학교 화학부, Korea
In this presentation, a systematic study of Seoul-Fluor will be presented. During our continuous efforts on the construction of drug-like small-molecule libraries using pDOS strategy, we aimed to develop a novel fluorescent core skeleton for the development of bioprobes, applicable for image-based screening. Guiding with computational simulation, we constructed a novel collection of fluorophores, which covers the full-color emission range with predictability. Seoul-Fluor analogs were successfully applied in HCS. Lastly, we developed a new target identification platform, FITGE, which aims to preserve protein-small molecule interactions under the intact cellular environment. After a series of failures using conventional target ID methods, we successfully identified the protein target of anti-proliferative compound with FITGE only under the live cell condition and observed the environment-dependent binding events of a functional small molecule by direct comparison between live cells and cell lysates. Even though it still requires the synthesis of bioactive probes with photo-crosslinker moiety, we believe our FITGE strategy can provide a unique technology platform for target identification in live cells.

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